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We report herein the cloning of the 127-kb entire gene cluster for desertomycin biosynthesis using bacterial artificial chromosome vector. The entire biosynthetic gene cluster for desertomycin was introduced in the heterologous host, Streptomyces lividans TK23, with an average yield of more than 130 mg l -1 .
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The Japanese Kanji for the "car" or "vehicle" is 車. As a standalone Kanji, it is pronounced as "Kuruma" but when it appears in combination with other Kanji, the pronunciation is "Sha" - for example, Densha (電車), meaning Train or Jitensha (自転車), meaning Bicycle. The Kanji 車 is constructed with 7 strokes. This.
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The classifiers we developed, using inferred labels obtained from patient subtypes showed that a support vector machine (SVM), gave the best classification results, with an accuracy of 0.82 with the test dataset. Using these subtypes, we ranked genes based on RNA expression levels. The top 25 genes were investigated, to elucidate the mechanisms.
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